實(shí)驗(yàn)地點(diǎn):肇慶市疾病預(yù)防控制中心
肇慶市疾病預(yù)防控制中心是原肇慶市衛(wèi)生防疫站以及肇慶市職業(yè)病防治院、端州、鼎湖、大旺高新區(qū)衛(wèi)生防疫站的基礎(chǔ)上組建而成,屬社會(huì)公益性衛(wèi)生事業(yè)單位。2002年12月26日掛牌成立。承擔(dān)著全市390萬人口的疾病預(yù)防控制、免疫規(guī)劃、職業(yè)病防治、衛(wèi)生監(jiān)測(cè)、衛(wèi)生檢驗(yàn)、健康教育、科研培訓(xùn)、突發(fā)公共衛(wèi)生事件應(yīng)急處理等工作職能,直管端州、鼎湖、大旺三區(qū)疾病控制和衛(wèi)生監(jiān)測(cè)、檢驗(yàn)工作,是全市疾病預(yù)防控制和衛(wèi)生檢測(cè)業(yè)務(wù)技術(shù)指導(dǎo)中心。
實(shí)驗(yàn):老鼠耳朵提RNA
老鼠耳朵研磨提取RNA,用于做后續(xù)基因、鼠疫病毒檢測(cè);因?yàn)槔鲜蠖浜?、毛發(fā)、皮膚,所以相對(duì)比較難磨,用戶之前用手工配合液氮研磨,如此一來樣本損失比較多,而且研磨過程中存在污染導(dǎo)致樣本活性降解。上海凈信全自動(dòng)樣品快速研磨儀可批量處理樣本,每個(gè)樣本都是獨(dú)立離心管,不會(huì)交叉污染,用戶可批量化抽提基因組,大大提高了工作效率。
實(shí)驗(yàn)設(shè)備:
品牌:上海凈信;名稱:全自動(dòng)樣品快速研磨儀;型號(hào):JXFSTPRP-24L
實(shí)驗(yàn)步驟:
準(zhǔn)備:待研磨老鼠耳朵樣本,離心管以及不銹鋼研磨珠若干顆,清洗并滅菌;金屬適配器放入-80度冰箱預(yù)冷凍10分鐘。
1、待研磨樣本裝入離心管,加入清洗并滅菌后的不銹鋼研磨珠,加入試劑
2、將裝有樣品離心管裝入上海凈信全自動(dòng)樣品快速研磨儀,注意水平配平
3、鎖緊鎖扣,設(shè)置參數(shù);
4、啟動(dòng)研磨,待研磨完成后取出樣品如下圖;

結(jié)語:用戶傳統(tǒng)的方式是手工研磨,因?yàn)闃颖倔w積小,手工研磨樣本損失比較嚴(yán)重,提出的RNA濃度非常低,所以用戶最終選擇上海凈信全自動(dòng)樣品快速研磨儀,樣本零損失,而且操作簡(jiǎn)單便捷,可以批量處理,用戶對(duì)上海凈信全自動(dòng)樣品快速研磨儀評(píng)價(jià)非常高。
儀器介紹:

上海凈信?的JXFSTPRP-L?系列組織研磨儀(專利產(chǎn)品,仿冒必究!ZL2019301918692)是一種特殊的、快速的、高效率的、多試管的一致系統(tǒng)。它能將任何來源(包括土壤、植物和動(dòng)物的組織/器官、細(xì)菌、酵母、真菌、孢子、古生物標(biāo)本等)的原始DNA、RNA和蛋白質(zhì)進(jìn)行提取和純化。 將樣品和研磨球放入研磨儀內(nèi)(配置研磨罐或離心管/適配器),在高頻擺動(dòng)作用下,研磨球在研磨儀內(nèi)來回高速撞擊及摩擦,在非常短的時(shí)間內(nèi)便可完成樣品的研磨、粉碎、混合及細(xì)胞破壁。
性能特點(diǎn):
1.穩(wěn)定性好:采用三維一體8字形振蕩方式,研磨更充分,穩(wěn)定性更好;
2.效率高:1分鐘內(nèi)完成2×24、2×48、4×96個(gè)樣品的研磨;
3.重復(fù)性好:同一組織樣本設(shè)定相同程序,獲得相同的研磨效果;
4.操作簡(jiǎn)便:內(nèi)置程序控制器,可對(duì)研磨時(shí)間、轉(zhuǎn)子的振動(dòng)頻率等參數(shù)進(jìn)行設(shè)置;
5.安全性高:具備安全罩及安全鎖;
6.無交叉污染:在研磨過程中處于全封閉狀態(tài),避免交叉污染;
7.低噪音:儀器運(yùn)行過程中,噪音小于55dB,不會(huì)對(duì)其它實(shí)驗(yàn)或儀器產(chǎn)生干擾。
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Vertical mixer
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Non-contact ultrasonic DNA interrupter
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Standard vacuum freeze dryer
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Orbital shaker
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Non-contact shaped DNA interrupter
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Rotary mixer HS-5
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Dual-channel non-contact energy-concentrated DNA interrupter
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Orbital shaker
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Roller mixer (blood mixer)
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Rotational viscometer
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Inching vortex oscillator
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Cup type ultrasonic cell crusher
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Micro centrifuge
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Multifunctional vortex oscillator
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Ultrasonic cell crusher integrated
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Ultrasonic crusher
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Snowflake ice maker
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Microplate centrifuge
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Nitrogen Blowing Apparatus
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Split ultrasonic cell disruptor
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Ultrasonic crusher
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Snowflake ice maker
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Ultrasonic cell crusher
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Nitrogen Blowing Apparatus
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Dual-channel non-contact energy-concentrated DNA interrupter
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Ultrasonic cell crusher
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Nitrogen Blowing Apparatus
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Visual nitrogen blowing instrument
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Intelligent ultrasonic crusher
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Ultrasonic cell crusher
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Constant temperature airtight ultrasonic reactor
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Automatic sample fast grinder-24L
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Planetary Ball Mill
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Infrared inoculation loop sterilizer
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Automatic sample fast grinder-96L
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Infrared inoculation loop sterilizer
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Hair drug detector
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Hair screening drug detector
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Biological indicator incubator
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Portable hair grinder
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Hair Grinding and Shredder
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Multi-sample tissue grinding machine-FEIII
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QuEChERS sample preparation system
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Multi-sample tissue grinder-32L
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Three-dimensional high-speed vibration ball mill
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Multi-sample tissue grinder-192L
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Three-head shock type high-energy ball mill
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Fast tissue low-temperature crushing homogenizer
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Multifunctional homogenizer
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Multi-sample tissue grinder-96L
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3D Centrifugal Cryogenic Grinder
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Vertical oscillator
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Constant temperature and humidity weighing system
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Water quality chlorophyll grinder
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Chlorophyll A grinder
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Mortar grinder
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High-efficiency vibrating disc grinder
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Fully automatic cryogenic sample processing system
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Automatic sample rapid grinder
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Multi-sample tissue grinder-64L
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Multi-channel microbial magnetic bead homogenizer
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Automatic sample rapid grinding machine-64
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Automatic sample rapid grinder-96
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Automatic sample rapid grinder-192
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Three-dimensional mixer
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Molecular hybridization furnace
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Cryogenic Grinder JXFSTPRP-CLN
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Cryogenic tissue grinder
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Cryogenic tissue grinder
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High-speed cryogenic grinding homogenizer
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Semi-automatic hammer cyclone mill
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High-speed tissue grinding machine KZ-II
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Low temperature hammer type cyclone mill
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Automatic sample rapid grinding machine
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Automatic sample rapid grinder-24
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QuEChERS pre-processing all-in-one machine
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Immersion liquid nitrogen Cryogenic Grinder
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Automatic liquid nitrogen freezing grinding machine
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Cryogenic Grinder (Semi-automatic Liquid Nitrogen Cryogenic Grinder)
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3D sample tissue grinder
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Cryogenic Grinding Machine
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Automatic hammer cyclone mill
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Multi-sample Tissue Grinder-24L
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Constant temperature mixer
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Beating sample homogenizer
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Beating aseptic homogenizer
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Jaw Crusher
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Automatic homogenizer
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Single cell suspension preparation instrument
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Single cell preparation instrument (temperature control)
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Biological indicator incubator
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Dry thermostat
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Knife grinder
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Constant temperature mixer (refrigeration)
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Microplate Heat Sealer
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Microplate Oscillator
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Oscillator special for microplate
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Microplate incubator
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Microplate incubator
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Thermostatic metal bath
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Temperature-controlled single cell suspension preparation instrument
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High-speed grinding homogenizer-192
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Bone and Teeth Grinder
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Frozen Bone Teeth Crusher
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Basic cryogenic grinder
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Cryogenic grinder
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Frozen grinder
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Cryogenic tissue grinder
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3D Centrifugal Cryogenic Grinder
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Cross-beating grinder
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Gene amplification instrument PCR instrument THT-96G (gradient type)
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Automatic rotating sampler
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Flip oscillator
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Fully Automatic Flip Oscillator
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Fully automatic low temperature flip oscillator
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Handheld Grinder
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High Power Handheld Grinder
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Parallel grinder
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Aquatic product animal husbandry solid phase extraction device
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Gradient amplification PCR detection analyzer THG-96G (gradient)
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3D Cryogenic Grinder
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Thermostatic metal bath refrigeration type
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Mini refrigerated metal bath
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High temperature four-module thermostatic metal bath
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Microplate Shaker
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High energy ball mill
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Low-Temperature Cyclone Hammer Mill
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Mini Diamond Grinding Machine
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MINI rapid detection grinder
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Portable sample grinder
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Small bead mill/cell bead mill
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Three-dimensional mixer
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Hot-lid metal bath
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High Throughput Tissue Grinder-2018
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Oil-free vacuum pump
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High temperature type CNC super constant temperature bath
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Solid Phase Extraction Apparatus
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Solid phase extraction device
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Grinding bead homogenizer
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Beating aseptic homogenizer
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Fully automatic homogenizer
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New type soil drying box
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High Throughput Tissue Grinder - GTL64
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In situ hybridization
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High Throughput Grinder
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Medium Flux Grinder
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High-throughput sample rapid grinder
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Ultra High Throughput Tissue Grinder
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Ultra High Throughput Tissue Grinder
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UV crosslinker
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Three-dimensional mixer
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Sample pre-processing machine
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